Magelan

Multiple Applications Gel Analyzer - Magelan

Overview

A Java-based image recognition tool to read and analyze images of electrophoretic gels for Allele-Calling, Fingerprinting, and Fragment-Sizing. A tutorial and whitepaper are available.

DNA Fingerprinting Mode

Fragment-sizing Mode

Genotyping and Allele-calling Mode

Features

Images

  • Accepts all common image formats such as gif, jpeg, and tif
  • Image manipulation tools such as rotating and adjusting the brightness and contrast
  • Views of the entire gel and specific lanes
  • Magnification for closer inspection
  • Automatic background subtraction

Interface and Setup

  • Simple, user-friendly
  • Easily adjustable threshold levels
  • Quick and easy set-up and storage of analysis templates, including standards information
  • Memory of user-defined standards
  • Flexible grid placement and shaping to account for experimental irregularities
  • FastFlow Buttons for streamlining the analysis
  • Easy ways to name samples or map wells to lanes

Analysis and Output

  • Fast and efficient algorithm analyzes gels in seconds
  • Adjustable thresholds for the entire gel, a particular tier or a specific lane
  • Histogram of intensities for a graphical interpretation of a lane image
  • Accurately identifies bands and genotypes
  • Color-coded genotypes for instant identification
  • Multiple output formats, such as tab-delimited file or plate representation

Windows and Unix versions

Additional Resources

Magelan White Paper

Magelan Tutorial